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. 2014 Nov 10;8:364. doi: 10.3389/fnins.2014.00364

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Copyright © 2014 Henson, Parupalli, Ju and Taylor.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The Et(cp:EGFP)^sj2 line expresses GFP in the diencephalic choroid plexus and myelencephalic choroid plexus. (A) Confocal image showing the dorsal view of the head of 3 dpf Et(cp:EGFP)^sj2 larvae expressing GFP in the diencephalic choroid plexus and myelencephalic choroid plexus. (B) Whole-mount immunohistochemical staining of Et(cp:EGFP)^sj2 larvae at 6 dpf. GFP in green labels the diencephalic choroid plexus and myelencephalic choroid plexus, and Zpr-1 shown in red labels the pineal gland and photoreceptors. The yellow overlay demonstrates the interaction between the pineal gland and diencephalic choroid plexus. The solid and dashed lines across the diencephalic choroid plexus and myelencephalic choroid plexus in the inset image indicate approximate regions where sections were taken for (C,D). (C) Transverse section through the diencephalic choroid plexus of 6 dpf Et(cp:EGFP)^sj2 larvae stained with GFP, DAPI, and Zpr-1. (D) Transverse section through the myelencephalic choroid plexus of 6 dpf Et(cp:EGFP)^sj2 larvae stained with GFP and DAPI. (E) Transverse section through the diencephalic choroid plexus of a 1-month-old Et(cp:EGFP)^sj2 zebrafish stained with GFP and Zpr-1 to label the diencephalic choroid plexus and pineal gland, respectively. (F) Transverse section through the myelencephalic choroid plexus of a 1-month-old Et(cp:EGFP)^sj2 zebrafish stained with GFP. Abbreviations: diencephalic CP, diencephalic choroid plexus; mCP, myelencephalic CP; GFP, green fluorescent protein; DAPI, 4′,6-diamidino-2-phenylindole; E, eye; F, forebrain; H, hindbrain. For all images: filled arrowhead, diencephalic choroid plexus; open arrowhead, pineal gland; arrow, myelencephalic choroid plexus. Scale bars are 50 μm.

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