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. 2014 Nov 10;8:364. doi: 10.3389/fnins.2014.00364

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Copyright © 2014 Henson, Parupalli, Ju and Taylor.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Rhodamine 123 and fluorescein ventricle injection demonstrates transporter activity in the CP. Dorsal fluorescent (A) and merged (bright-field and Rhodamine 123) (B) confocal image of 4 dpf Casper larvae with Rhodamine 123 (red) injection into the brain ventricle. The rhodamine 123 concentrates within the myelencephalic choroid plexus (arrowhead). Rhodamine 123 also fluoresces green and co-localizes with red emission (not shown). Lateral fluorescent (C) and merged (bright-field and Rhodamine 123) (D) confocal image of 4 dpf Casper larvae with Rhodamine 123 (red) injection into the brain ventricle. The rhodamine 123 concentrates within the myelencephalic choroid plexus (arrowhead) and diencephalic choroid plexus (arrow). Dorsal fluorescent (E) and merged (bright-field and fluorescein) (F) confocal image of 4 dpf Casper larvae with fluorescein (green) injected into the brain ventricle. Fluorescein concentrates within the myelencephalic choroid plexus (arrowhead) and diencephalic choroid plexus (arrow). Lateral fluorescent (G) and merged(bright-field and fluorescein) (H) confocal image of 4 dpf Casper larvae with fluorescein (green) injected into the brain ventricle. Fluorescein concentrates within the myelencephalic choroid plexus (arrowhead) and diencephalic choroid plexus (arrow). Abbreviations: mCP, myelencephalic choroid plexus; dCP, diencephalic choroid plexus. Scale bar is 50 μm.

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