Table 2.
Characterization of Et(cp:EGFP) mutants generated from the forward genetic screen.
| Class | Allele | Map position | DPF | Other phenotypes(4 dpf) | Fluorescein transport | Onset of brain cell death (up to 4 dpf) | Initial circulation loss (up to 4 dpf) |
|---|---|---|---|---|---|---|---|
| I: reduced GFP expression, dispersed epithelia, and irregularly shaped CP | cp26.3a | ND | 2–3 | No swimbladder; brain and tail cell death; brain ventricle hemorrhaging; faint heartbeat; no circulation; no midbrain-hindbrain boundaries | Accumulates within third ventricle | 3 dpf | 3 dpf |
| cp140.2 | ND | 2 | Small head; small eyes; no swimbladder; heart edema; brain cell death; faint heartbeat and circulation; slightly larger third ventricle and wider lateral ventricles ventricle | Increased uptake; accumulates within brain ventricle | 4 dpf | 4 dpf | |
| II: normal GFP expression, smaller epithelial aggregates, and irregularly shaped CP | cp9.6 | LG 4 | 2 | Small head; small eyes; no swimbladder; heart edema; occasional hydrocephalus; brain cell death; no circulation; no distinct third ventricle or lateral ventricles and severely reduced fourth ventricle | Increased uptake; accumulates within brain ventricle | 2 dpf | 4 dpf |
| cp79.6 | ND | 2 | Small head; small eyes; no swimbladder; heart edema; many still in chorion; brain cell death; hemorrhaging around heart; slow heartbeat; little to no circulation; wider lateral ventricles | Dispersed punctate staining on the dorsal ventricle surface | 4 dpf | 3 dpf | |
| III: normal GFP expression, smaller epithelial aggregates, and expanded CP | cp79.8 | ND | 2 | Small head; small eyes; no swimbladder; brain cell death; hemorrhaging around heart; decreased heartbeat and circulation; wider lateral ventricles | Increased uptake; accumulates within brain ventricle | 4 dpf | 3 dpf |
| cp151.2 | ND | 2–3 | Small head; small eyes; no swimbladder; brain cell death; minor swelling around head; occasional hemorrhaging around heart; smaller third ventricle and no defined lateral ventricles | Increased uptake; accumulates within brain ventricle | 4 dpf | 4 dpf | |
| IV: variable GFP expression, smaller epithelial aggregates, and expanded CP | cp27.5 | LG 21 | 2 | Small head; small eyes; no swimbladder; brain cell death; heart edema; small arched body; curved tail; hydrocephalus; occasional hemorrhaging in brain ventricle; heartbeat, but little to no circulation; no defined ventricle boundaries | Increased uptake; accumulates within brain ventricle | 2 dpf (localized to forebrain) | 3 dpf |
| cp44.10 | ND | 2 | Small head; small eyes; no swimbladder; brain cell death; heart edema; small arched body; curved tail; hydrocephalus; occasional hemorrhaging around heart; heartbeat, but little to no circulation; no third ventricle or lateral ventricles and irregularly shaped fourth ventricle | Increased uptake; accumulates within brain ventricle | 2 dpf (localized to forebrain) | 4 dpf | |
| V: normal GFP expression, compact epithelia, and enlarged CP | cp26.6b | ND | 4 | Rounded head, protruding lower jaw; no swimbladder; edema around heart, eyes, and gut; faint heartbeat and circulation; normal brain ventricle | N/A | N/A | 4 dpf |
| cp105.2b | ND | 4–5 | Rounded head, protruding lower jaw; no swimbladder; severe edema around heart, eyes, and gut; faint heartbeat and circulation; slightly smaller fourth ventricle | N/A | N/A | 6 dpf |
a
Observations recorded at 3 dpf;
b
observations recorded at 5 dpf;
ND, not determined; N/A, not applicable.