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. 2014 Nov 10;5:620. doi: 10.3389/fpls.2014.00620

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Copyright © 2014 Mouradov and Spangenberg.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phenotypes (A) and biochemical profiles (B) of TrANRhp transgenic white clover lines with down-regulated TrANR gene (Abeynayake et al., 2012). (A): (a) wild-type white clover inflorescence; (b) wild-type flowers at different developmental stages; (c) TrANRhp inflorescence; (d) TrANRhp flowers at different developmental stages; (e) wild-type mature flower; (f) wild-type carpel; (g) cross-section of a carpel stained with DMACA; (h) epidermal cells of anther filaments stained with DMACA; (i) TrANRhp mature flower; (j) TrANRhp carpel; (k) cross-section of TrANRhp carpel; (l) epidermal cells of TrANRhp anther filaments. (B) level and composition of flavan 3-ols; (C) level and composition of anthocyanins. Bars = 2 mm (a–d); 1 mm (e,f,i,j); 500 μm (g,k) and 75 μm (h,l). GC, gallocatechin; EGC, epigallocatechin; A1, delphinidin 3-sambubioside; A2, cyanidin 3-sambubioside.