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. 2014 Sep 24;166(3):1463–1478. doi: 10.1104/pp.114.246769

Figure 7.

Figure 7.

GSD1 is localized to PM and PD. A to D, GSD1 fused to GFP (GFP-GSD1) was expressed in tobacco leaf epidermal cells. GFP-GSD1 fluorescence detected on PM (A), callose staining with aniline blue indicating PD sites (B), and merged image showing that GSD1 localizes to PM and PD (C). Arrowhead indicates PD sites on the PM. D, GFP-GSD1 fluorescence detection after plasmolysis. E and F, Control GFP was expressed in onion epidermal cells. Control free GFP fluorescence signal (E). GFP control fluorescence merged with DIC image (F). G to I, GSD1 fused with GFP (GFP-GSD1) was expressed in onion epidermal cells. GFP-GSD1 fluorescence on the PM (G), GFP-GSD1 fluorescence merged with the DIC image (H), and image after plasmolysis treatment (I). J to L, Representative immunogold labeling of GSD1 and quantitative analysis of gold particles. GSD1 is localized to the channel of PD (J) and at the neck regions of PD (K) of the companion cells in 4-week-old rice shoot tissues. Quantification of gold particles is depicted in L. Arrowheads indicate immunogold labeling particles. CC, Companion cell; CW, cell wall; DIC, differential interference contrast; HS, Hechtian strand; N, nucleus; SE, sieve element. Bars = 50 μm in A to C and E to I and 500 nm in J and K.