pex2-1 and pex10-2 mutants display IBA resistance and PTS2 processing defects. A, Root lengths of 8-d-old wild-type (Col-0) or pex seedlings grown in yellow-filtered light in the presence or absence of Suc or on Suc-supplemented medium containing 10 µm
IBA are shown. Error bars show sds of the means (n ≥ 12). Different letters above bars represent significantly different means (one-way ANOVA, P < 0.005). B, Hypocotyl lengths of 6-d-old wild-type (Col-0) or pex seedlings grown in the dark in the presence or absence of Suc or on Suc-supplemented medium containing 30 µm
IBA are shown. Error bars show sds of the means (n ≥ 13). Different letters above bars represent significantly different means (one-way ANOVA, P < 0.005). C, Lateral roots per millimeter of root length of 7-d-old wild-type (Col-0) or pex seedlings 3 d after transfer to Suc-containing medium with or without 10 µm
IBA are shown. Error bars show sds of the means (n ≥ 8). Different letters above bars represent significantly different means (one-way ANOVA, P < 0.005). D, Protein extracts from the 8-d-old seedlings grown in the light on 0.5% Suc from A were processed for immunoblotting. The membrane was serially probed with antibodies to the indicated proteins. The positions of molecular mass markers (in kilodaltons) are indicated on the right. PMDH is synthesized as a precursor (p) containing the PTS2 signal that is processed into the mature (m) protein in the peroxisome. HSC70 was used to monitor loading. E, Protein extracts from 4-d-old seedlings grown in the light on 0.5% Suc were processed for immunoblotting and probed with the indicated antibodies. HSC70 was used to monitor loading. Experiments in A to E were repeated at least three times with similar results. *, A cross-reacting band that appears with the PEX10 antibody.