Figure 7.

PEX5 overexpression alters pex10-2 defects but does not notably impact pex2-1. A, Hypocotyl lengths of 7-d-old wild-type (Wt; Col-0) or pex seedlings without or with PEX5 overexpression (35S:PEX5) grown in the dark in the presence or absence of Suc or on Suc-supplemented medium containing 30 µm IBA are shown. Error bars show sds of the means (n ≥ 22). Different letters above bars represent significantly different means (one-way ANOVA, P < 0.005). B, Lateral roots per millimeter of root length of 7-d-old seedlings 3 d after transfer to Suc-containing medium with or without 10 µm IBA are shown. Error bars show sds of the means (n ≥ 8). Different letters above bars represent significantly different means (one-way ANOVA, P < 0.005). C, Protein extracts from 4-, 5-, and 6-d-old seedlings grown in the light on 0.5% Suc were processed for immunoblotting. Membranes from duplicate gels were serially probed with antibodies to the indicated proteins. *, A cross-reacting band that appears with the ICL antibody. The positions of molecular mass markers (in kilodaltons) are indicated on the right. PMDH and thiolase are synthesized as precursor (p) containing the PTS2 signal that is processed into the mature (m) protein in the peroxisome. HSC70 was used to monitor loading. These experiments were repeated twice with similar results.