Figure 8.

pex2-1 restores PEX5 protein levels to pex6-1 but not pex7-1. A, Root lengths of 8-d-old wild-type (Wt; Col-0) or pex seedlings grown in yellow-filtered light in the presence of Suc or on Suc-supplemented medium containing 10 µm IBA are shown. Error bars show sds of the means (n ≥ 18). Different letters above bars represent significantly different means (one-way ANOVA, P < 0.005). B, Root lengths of 8-d-old seedlings grown in yellow-filtered light in the presence or absence of Suc. Error bars show sds of the means (n ≥ 16). Different letters above bars represent significantly different means (one-way ANOVA, P < 0.005). C, Photographs of 8-d-old seedlings from B. Bar = 1 cm. D, Protein extracts from 8-d-old seedlings grown in the light on 0.5% Suc from A were processed for immunoblotting. The membrane was serially probed with antibodies to the indicated proteins. PMDH and thiolase are synthesized as precursors (p) containing the PTS2 signal that is processed into the mature (m) protein in the peroxisome. Numbers below the PMDH or thiolase bands indicate the percentages remaining unprocessed in each lane. HSC70 was used to monitor loading, and numbers below PEX5 and PEX7 bands indicate ratios of the band to HSC70 that are normalized such that the ratio from the wild type was set to 1.0. These experiments were repeated three times with similar results.