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. 2014 Sep 9;166(3):1560–1574. doi: 10.1104/pp.114.247866

Table I. Leaf and photosynthetic characteristics of wild-type N. tabacum, two knockdown lines (RI9 and RI29) with reduced AOX, and an AOX-overexpressing line (B7).

All analyses used the fourth or fifth fully developed leaves of well-watered or moderate drought-stressed plants. The ΦPSII, gs, gm, Ci, and Cc values were measured at saturating irradiance (1,600 PPFD) and Ca of 400 µmol mol−1. Results are the mean ± se of at least three independent experiments. Within each treatment, plant lines not sharing a common superscript letter are significantly different from one another (P < 0.05).

Parameter Well-Watered Plants
Moderate Drought-Stressed Plants
B7 Wild Type RI9 RI29 B7 Wild Type RI9 RI29
Leaf RWC (%) 87 ± 2a 89 ± 2a 87 ± 2a 88 ± 3a 76 ± 1a 77 ± 3a 78 ± 2a 77 ± 1a
Fv/Fm 0.81 ± 0.009a 0.80 ± 0.007a 0.82 ± 0.009a 0.81 ± 0.003a 0.79 ± 0.012a 0.80 ± 0.007a 0.81 ± 0.006a 0.80 ± 0.012a
ΦPSII 0.211 ± 0.014a 0.221 ± 0.013a 0.220 ± 0.017a 0.208 ± 0.018a 0.156 ± 0.010a 0.141 ± 0.060a 0.100 ± 0.011b 0.089 ± 0.011b
gs (mol m−2 s−1) 0.150 ± 0.015a 0.160 ± 0.012a 0.143 ± 0.014a,b 0.126 ± 0.015b 0.100 ± 0.006a 0.103 ± 0.009a 0.090 ± 0.005a 0.087 ± 0.007a
gm (mol m−2 s−1) 0.115 ± 0.003a 0.121 ± 0.004a 0.113 ± 0.005a 0.121 ± 0.013a 0.113 ± 0.004a 0.111 ± 0.005a 0.110 ± 0.008a 0.119 ± 0.008a
Ci (µmol mol−1) 309 ± 14a 320 ± 10a 318 ± 13a 263 ± 8b 212 ± 11a 201 ± 16a 189 ± 5a 196 ± 9a
Cc (µmol mol−1) 227 ± 8a,b 240 ± 9a 237 ± 8a 194 ± 9b 160 ± 10a 154 ± 9a 156 ± 18a 150 ± 8a
CE (CO2 m−2 s−1/mol−1 CO2) 0.071 ± 0.001a 0.066 ± 0.003a 0.073 ± 0.005a 0.061 ± 0.007a 0.046 ± 0.003a 0.051 ± 0.008a 0.039 ± 0.006a 0.030 ± 0.003b
Total Chl (mg m−2 leaf area) 334 ± 35a 320 ± 36a 337 ± 26a 331 ± 20a 300 ± 33a 288 ± 31a,b 259 ± 29a,b 248 ± 16b
Chl a (mg m−2) 223 ± 19a 231 ± 23a 235 ± 12a 234 ± 14a 202 ± 16a 196 ± 15a,b 165 ± 13b,c 154 ± 10c
Chl b (mg m−2) 111 ± 16a 89 ± 13a 102 ± 14a 97 ± 8a 97 ± 17a 93 ± 17a 94 ± 16a 94 ± 14a
Chl a/b (ratio) 2.18 ± 0.14b 2.63 ± 0.15a 2.35 ± 0.21a,b 2.44 ± 0.16a,b 2.19 ± 0.27a 2.20 ± 0.25a 1.81 ± 0.18b 1.73 ± 0.33b
Protein (g m−2 leaf area) 2.82 ± 0.12a 2.94 ± 0.10a 2.97 ± 0.06a 2.85 ± 0.09a 2.63 ± 0.15a 2.55 ± 0.06a,b 2.29 ± 0.13b 2.40 ± 0.19a,b