Table I. Leaf and photosynthetic characteristics of wild-type N. tabacum, two knockdown lines (RI9 and RI29) with reduced AOX, and an AOX-overexpressing line (B7).
Parameter | Well-Watered Plants |
Moderate Drought-Stressed Plants |
||||||
---|---|---|---|---|---|---|---|---|
B7 | Wild Type | RI9 | RI29 | B7 | Wild Type | RI9 | RI29 | |
Leaf RWC (%) | 87 ± 2a | 89 ± 2a | 87 ± 2a | 88 ± 3a | 76 ± 1a | 77 ± 3a | 78 ± 2a | 77 ± 1a |
Fv/Fm | 0.81 ± 0.009a | 0.80 ± 0.007a | 0.82 ± 0.009a | 0.81 ± 0.003a | 0.79 ± 0.012a | 0.80 ± 0.007a | 0.81 ± 0.006a | 0.80 ± 0.012a |
ΦPSII | 0.211 ± 0.014a | 0.221 ± 0.013a | 0.220 ± 0.017a | 0.208 ± 0.018a | 0.156 ± 0.010a | 0.141 ± 0.060a | 0.100 ± 0.011b | 0.089 ± 0.011b |
gs (mol m−2 s−1) | 0.150 ± 0.015a | 0.160 ± 0.012a | 0.143 ± 0.014a,b | 0.126 ± 0.015b | 0.100 ± 0.006a | 0.103 ± 0.009a | 0.090 ± 0.005a | 0.087 ± 0.007a |
gm (mol m−2 s−1) | 0.115 ± 0.003a | 0.121 ± 0.004a | 0.113 ± 0.005a | 0.121 ± 0.013a | 0.113 ± 0.004a | 0.111 ± 0.005a | 0.110 ± 0.008a | 0.119 ± 0.008a |
Ci (µmol mol−1) | 309 ± 14a | 320 ± 10a | 318 ± 13a | 263 ± 8b | 212 ± 11a | 201 ± 16a | 189 ± 5a | 196 ± 9a |
Cc (µmol mol−1) | 227 ± 8a,b | 240 ± 9a | 237 ± 8a | 194 ± 9b | 160 ± 10a | 154 ± 9a | 156 ± 18a | 150 ± 8a |
CE (CO2 m−2 s−1/mol−1 CO2) | 0.071 ± 0.001a | 0.066 ± 0.003a | 0.073 ± 0.005a | 0.061 ± 0.007a | 0.046 ± 0.003a | 0.051 ± 0.008a | 0.039 ± 0.006a | 0.030 ± 0.003b |
Total Chl (mg m−2 leaf area) | 334 ± 35a | 320 ± 36a | 337 ± 26a | 331 ± 20a | 300 ± 33a | 288 ± 31a,b | 259 ± 29a,b | 248 ± 16b |
Chl a (mg m−2) | 223 ± 19a | 231 ± 23a | 235 ± 12a | 234 ± 14a | 202 ± 16a | 196 ± 15a,b | 165 ± 13b,c | 154 ± 10c |
Chl b (mg m−2) | 111 ± 16a | 89 ± 13a | 102 ± 14a | 97 ± 8a | 97 ± 17a | 93 ± 17a | 94 ± 16a | 94 ± 14a |
Chl a/b (ratio) | 2.18 ± 0.14b | 2.63 ± 0.15a | 2.35 ± 0.21a,b | 2.44 ± 0.16a,b | 2.19 ± 0.27a | 2.20 ± 0.25a | 1.81 ± 0.18b | 1.73 ± 0.33b |
Protein (g m−2 leaf area) | 2.82 ± 0.12a | 2.94 ± 0.10a | 2.97 ± 0.06a | 2.85 ± 0.09a | 2.63 ± 0.15a | 2.55 ± 0.06a,b | 2.29 ± 0.13b | 2.40 ± 0.19a,b |