Figure 6.

Indaziflam treatment decreased the net colocalization between MTs and YFP:CESA6 at the PM. Arabidopsis seedlings expressing both RFP:TUA5 and YFP:CESA6 in prc1-1 were grown in the dark for 3 d before imaging. Representative single optical sections (monochrome) of cortical MTs labeled by RFP:TUA5 (magenta) and PM-localized YFP:CESA6 (green) were used for the colocalization analysis (Table I). After 2 h in 0.01% DMSO mock, 71% ± 1% of YFP:CESA6 particles were coaligned with MTs, which was not different from the ratio without any treatment (Li et al., 2012). After 2 h in 500 nm indaziflam, the colocalization ratio between YFP:CESA6 and RFP:TUA5 decreased to 53% ± 4%, which was not significantly different from the expected random ratio association of 47% ± 10%. Bar = 5 μm.