Figure 7.

GUDK regulates the expression of OsAP37 target genes. A, qPCR analysis of OsAP37 downstream target genes in the wild type and gudk-1 and gudk-2 insertion mutant lines under well-watered as well as drought stress conditions, using target gene primers (Supplemental Table S1D). Gene expression was normalized with reference to Ubiquitin and is expressed as the fold change in drought stress over well-watered samples. B, Dual firefly-renilla luciferase transactivation assays with reporter (target promoters fused to firefly luciferase), effector-1 (CaMV 35S:OsAP37), and effector-2 (CaMV 35S:GUDK) plasmids transfected into rice protoplasts along with renilla luciferase plasmid as the internal control. Results are expressed as the ratio of luminescence obtained from firefly luciferase to the luminescence obtained from renilla luciferase (FLuc/RLuc). The data points are the means ± se of three independent experiments with significance tested at P ≤ 0.01 compared with the relevant controls. WT, Wild type. [See online article for color version of this figure.]