Table I. Target proteins selected for in vitro phosphorylation assays.
The Mascot score, relative intensity, and number of peptides with percentage of sequence coverage are taken directly from the Mascot report after a rice genome database search using Mascot. The Mascot score represents those proteins that were significantly represented in the ion spectrum after fragmentation with a score > 40 (P < 0.05). Relative intensity is the measure of total concentration of the protein in the sample that is directly related to the number of peptides eluting from the HPLC column and plotted against time. —, Nondetection of protein in the sample.
| Proteins Identified by LC-MS/MS | Locus ID | Predicted Molecular Mass | Mascot Score |
Relative Intensity |
Peptides
(Sequence Coverage) |
|||
|---|---|---|---|---|---|---|---|---|
| Well Watered | Drought Stress | Well Watered | Drought Stress | Well Watered | Drought Stress | |||
| kDa | n (%) | |||||||
| Chlorophyll a/b-binding preprotein | Os01g52240 | 28.04 | — | 95.45 | — | 58,260 | — | 8 (23.65) |
| Granule-bound starch synthaseI | Os06g04200 | 66.99 | 46.78 | — | 50,471 | — | 7 (17.62) | — |
| Putative chloroplast phosphoglycerate kinase | Os01g58610 | 32.48 | 178.81 | 234.54 | 206,862 | 221,079 | 14 (32.08) | 16 (38.78) |
| AP2-1 protein (OsAP37) | Os01g58420 | 48.09 | 52.36 | 64.98 | 87,345 | 108,765 | 8 (28.78) | 12 (36.87) |
| Wall-associated kinase 4-like | Os01g49529 | 77.7 | 114.54 | 125.98 | 69,321 | 88,076 | 9 (18.54) | 12 (24.67) |
| Stress-induced receptor-like kinase1 | Os07g08860 | 57.7 | — | 99.77 | — | 82,349 | — | 9 (28.07) |
| αNAC-like protein | Os01g50360 | 93.5 | 79.02 | — | 89,095 | — | 9 (16.3) | — |
| GLABRA2-type homeodomain protein | Os04g48070 | 57.1 | 55.12 | — | 54,389 | — | 7 (19.67) | — |
| GA insensitive (GAI), repressor of GAI and scarecrow family transcription factor | Os11g31100 | 87.6 | 58.86 | — | 61,274 | — | 7 (19.85) | — |
| Myeloblastosis (MYB)-LIKE DNA-binding domain | Os03g51220 | 81.5 | 48.82 | — | — | 59,315 | — | 7 (21.20) |
| Putative F-box protein | Os02g10700 | 61.16 | 71.15 | — | — | 75,715 | — | 8 (18.03) |
| Basic helix-loop-helix protein-like | Os02g48060 | 30.82 | 42.45 | — | — | 215,417 | — | 13 (19.46) |
| Zinc finger CCCH domain-containing protein | Os02g35150 | 78.63 | 69.27 | — | 54,583 | — | 6 (19.81) | — |
| GA- and ABA-regulated MYB transcription factor | Os01g59660 | 60.79 | 43.21 | — | — | 53,567 | — | 8 (24.98) |