Figure 2.

CRISPR/Cas-induced mutations in a gene family. A, Schematic illustrating two sgRNAs (red arrows) targeting the Solyc08g041770 coding sequence and PCR and sequencing results showing indels in six of eight T0 plants. Plant 1 was chimeric for the desired deletion (1L), another allele (1S) carrying a 1-bp insertion in the target of sgRNA1 (blue arrow), and a 1-bp deletion in the target of sgRNA2. Plant 6 was also chimeric. B, Schematic illustrating two sgRNAs (red arrows) targeting the conserved second to last exon of the Solyc07g021170 and Solyc12g044760 homologs. An alignment of the targeted regions is presented below, and PCR genotyping is shown to the right. Asterisks indicate identical nucleotides. Four of eight T0 plants (red font), which were derived from the same callus, were homozygous for deletions in each gene. Note also the indels of various sizes in four additional chimeric plants.