Fatty acid (FA) quantification in seedlings. Percentage of FAs remaining is shown for 3-d (A), 5-d (B), and 7-d (C) etiolated seedlings grown on 0.8% (w/v) agar plates compared with the level in seeds. The amount of FA in dry seeds was set as 100%. Data represent means ± se of three independent experiments. For each experiment, n ≥ 50. *, Statistically significant difference of P < 0.01 from the wild type (Col-0, Col-3, or Ws-4); **, statistically significant difference of P < 0.001 from the wild type (Col-0, Col-3, or Ws-4); #, mutants in Ws-4 background.