Table I. Confirmed homozygous peroxisomal mutants analyzed in this study.
Based on the level of the full-length target transcripts, mutants were considered to be knockout (KO), knockdown (KD), or overexpressor (OE). β-oxidation assays were Suc dependence (SC), 2,4-DB (DB), OPDA, detection of oil bodies using Nile Red (NR), or fatty acids quantification (FA). ABA, Seed germination in the presence of ABA; D/L, requires light for complete germination; N/A, not applicable; X, mutant exhibits a phenotype; —, mutant has no phenotype.
| Gene Locus | Acronym | Annotation | PTS | Allele | Line | Ecotype | Level of Target Transcripts | β-Oxidation Processes | D/L | ABA | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| KO | KD | OE | SC | DB | OPDA | NR | FA | |||||||||
| At1g01710 | ACH2 | Acyl-CoA thioesterase2 | SKL | ach2-1 | SALK_134567 | Col-0 | X | — | X | X | — | X | — | X | ||
| ach2-2 | GK_705E01 | Col-0 | X | — | X | X | — | X | — | X | ||||||
| ach2-3 | SALK_126817C | Col-0 | X | — | — | X | X | X | X | X | ||||||
| At1g16730 | UP6 | Unknown protein6 | CRL | up6-1 | SALK_122395 | Col-0 | X | — | X | X | — | N/A | X | X | ||
| At1g19570 | DHAR1 | Dehydroascorbate reductase1 | dhar1-1 | SALK_005382 | Col-0 | X | — | X | — | — | N/A | X | X | |||
| dhar1-2 | SALK_029966C | Col-0 | X | — | X | — | — | N/A | X | X | ||||||
| At1g20560 | AAE1 | Acyl-activating enzyme isoform1 | SKL | aae1-1a | SALK_041152 | Col-0 | X | — | — | X | X | X | X | X | ||
| At1g48320 | DHNAT1/sT1 | DHNA-CoA thioesterase1/small thioesterase1 | AKL | dhnat1-1b | CS846230 | Col-0 | X | — | X | X | — | N/A | X | X | ||
| dhnat1-2 | SALK_066477C | Col-0 | X | — | — | — | — | N/A | X | X | ||||||
| At1g50510 | INDA | Indigoidine synthase A | RIx5HL | inda1 | GK_681B01 | Col-0 | X | X | — | — | X | X | X | X | ||
| At1g54340 | ICDH/IDHP1 | NADP-dependent isocitrate deshydrogenase | SRL | icdh1 | SALK_034151 | Col-0 | X | X | X | — | — | N/A | X | X | ||
| At1g64850 | CEF | Calcium binding EF-hand | SRL | cef-1 | CS806024 | Col-3 | X | — | — | — | N/A | N/A | N/A | N/A | ||
| At1g77540 | ATF2 | Acetyltransferase2 | SSI | atf2-1 | FLAG_206E09 | Ws-4 | X | — | — | — | N/A | N/A | N/A | N/A | ||
| At2g29590 | sT5 | Small thioesterase5 | SKL | st5-1 | SALK_089257 | Col-0 | X | — | — | X | — | N/A | X | X | ||
| st5-2 | SALK_092512 | Col-0 | X | — | — | X | — | N/A | X | X | ||||||
| At2g42490 | CuAO3 | Copper amine oxidase3 | SKL | cuao3-1 | SALK_095214 | Col-0 | X | — | — | X | — | N/A | X | — | ||
| cuao3-2 | SALK_096065C | Col-0 | X | — | — | — | — | N/A | X | — | ||||||
| At3g12800 | SDRb/DECR | Short-chain dehydrogenase/reductase isoform b/2,4-dienoyl-CoA reductase | SKL | decr-2 | CS877641 | Col-0 | X | — | X | — | — | N/A | X | X | ||
| decr-3 | CS871159 | Col-3 | X | — | X | — | — | N/A | X | X | ||||||
| At3g14150 | HAOX2 | Hydroxy-acid oxidase isoform2 | SML | haox2-1b | SALK_102409C | Col-0 | X | — | — | — | N/A | N/A | N/A | N/A | ||
| At3g48140 | B12D1 | Senescence-associated protein/B12D-related protein | b12d1-1 | FLAG_548C12 | Ws-4 | X | — | X | — | X | X | X | — | |||
| At3g48170 | BADH | Betaine Aldehyde deshydrogenase | SKL | badh1 | CS822971 | Col-0 | X | — | X | — | X | X | X | X | ||
| At3g55640 | CADC | Ca2+-dependent carrier | cadc1 | CS832927 | Col-0 | X | — | X | — | — | N/A | X | X | |||
| cadc2 | GK_237H07 | Col-0 | X | — | X | — | — | N/A | X | X | ||||||
| At3g56460 | ZnDH | Zinc-binding dehydrogenase | SKL | zndh1 | SALK_056640 | Col-0 | X | — | — | — | N/A | N/A | N/A | N/A | ||
| zndh2 | SALK_082243 | Col-0 | X | — | X | — | N/A | N/A | N/A | N/A | ||||||
| At4g02340 | EH3 | Epoxide hydrolase isoform3 | ASL | eh3-1 | SALK_106529C | Col-0 | X | — | — | X | — | N/A | X | X | ||
| eh3-2 | SALK_149885C | Col-0 | X | — | — | — | — | N/A | X | X | ||||||
| At4g04320 | MCD | Malonyl-CoA decarboxylase | SRL | mcd1 | SALK_069574C | Col-0 | X | — | — | — | N/A | N/A | N/A | N/A | ||
| mcd2 | GK_859E12 | Col-0 | X | — | — | — | N/A | N/A | N/A | N/A | ||||||
| At4g09320 | NDPK1 | Nucleoside diphosphate kinase type1 | ndpk1-1 | SALK_089749 | Col-0 | X | — | — | — | N/A | N/A | N/A | N/A | |||
| At5g04870 | CPK1 | Calcium-dependent protein kinase1 | cpk1-1b | SALK_007698 | Col-0 | X | — | X | X | — | N/A | — | — | |||
| cpk1-2b | SALK_080155 | Col-0 | X | — | X | — | — | N/A | — | — | ||||||
| At5g11910 | ELT1 | Esterase/lipase/thioesterase family isoform1 | SRI | elt1-1 | FLAG_632F03 | Ws-4 | X | — | X | — | — | N/A | X | X | ||
| At5g16370 | AAE5 | Acyl-activating enzyme5 | SRM | aae5-1a | SALK_009731 | Col-0 | X | — | X | — | X | X | X | |||
| At5g44250 | UP5 | Unknown protein5 | SRL | up5-1 | SALK_107281 | Col-0 | X | X | X | — | X | X | X | X | ||
| At5g47720 | ACAT1.3/AACT1 | Acetoacetyl-CoA thiolase1.3 | SAL | aact1-2b | SALK_008505 | Col-0 | X | — | X | — | — | N/A | X | X | ||
| At5g58220 | TTL1 | Transthyretin-like protein1 | RLx5HL | ttl3 | SALK_137289 | Col-0 | X | — | — | — | N/A | N/A | N/A | N/A | ||
| At5g65400 | UP7 | Ser Hydrolase1 | SLM | up7-1 | SALK_062218C | Col-0 | X | X | — | — | — | N/A | X | X | ||
| up7-2 | SALK_067081 | Col-0 | X | — | — | — | — | N/A | X | — | ||||||