Figure 7.

A, Schematic representation of the 1,329-bp intergenic region between AtClpB-C and AtCK2 genes with point of T-DNA insertion (in Salk_014505 mutant) and HSE distribution. The 4,393-bp T-DNA is inserted within the core promoter region. Of the four HSEs, the distal one (considering upstream of the AtClpB-C gene) is separated from the other three by T-DNA insertion. The configuration of the one HSE present within the 1kb promoter region of the AtCK2 gene and the three HSEs within the 1kb promoter region of AtClpB-C gene is shown. B, sqRT-PCR analysis of the mutant plants for expression of AtCK2 and AtClpB-C genes under HS of 38°C for 15 and 30 min. Three homozygous lines (named as 1, 2, and 3), one heterozygous line (4), and wild-type plants were analyzed. WT, Wild type. [See online article for color version of this figure.]