Figure 1.

Characterization of TALEN-induced mutations. A, PCR screen for TALEN-induced mutations in βE-pTAL425/6 M0 plants. Wild-type (WT) PRO amplicons were cleaved by Sm1I, producing 647- and 225-bp products, but the mutant amplicons in lines 1 to 5, 7, and 8 are not cleaved. Note that line 9 failed to amplify. The mutant line designations below the image correspond to the DNA sequences in B. M indicates the marker lane, and C indicates the no-template control. B, DNA sequence alignment of TALEN-induced mutations. Left and right TALEN-binding sequences are underlined in the wild type. The sizes of deletions (−) and insertions (+) are indicated to the right of the sequences. The red dashes indicate the location of a 39-bp insertion in pro_1.