Table I. Effect of PTN1 and PTN4 on Pi absorption is similar in a wide range of Pi concentration.
Plants were grown for 5 d in complete medium (full) then transferred for 5 d in low Pi with or without (Control) drugs (at optimal concentration). Pi influx was measured during 2 h in 33P supplemented with 5, 25, 50, 100, 500, and 1,000 μm Pi for the measure of Vmax and Km. At least 10 plants were used for each point.
Transporter | Control |
PTN1 |
PTN4 |
|||
---|---|---|---|---|---|---|
Vmax | Km | Vmax | Km | Vmax | Km | |
High affinity | 0.14 | 9.4 | 0.12 | 13.3 | 0.09 | 8.2 |
Low affinity | 0.37 | 104.3 | 0.27 | 91.6 | 0.3 | 108.6 |