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. 2014 Oct 20;3:22. doi: 10.1186/2047-217X-3-22

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Copyright © 2014 Quick et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The top row plots demonstrate collector’s curves of sequence reads over time measured in hours for the R7 (left) and R7.3 (right) run. Visible on the R7 run is a change in rate of read acquisition associated with topping-up the flowcell with additional library at hours 12, 24, 36 and 48. For the R7.3 run, the updated MinKNOW software reselects sequencing wells after 24 hours. The bottom row shows the histogram of read counts for reads shorter than 50,000 bp in length for the R7 (left) and R7.3 (right) run.