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. Author manuscript; available in PMC: 2015 Nov 12.
Published in final edited form as: Tetrahedron Lett. 2014 Sep 28;55(46):6311–6314. doi: 10.1016/j.tetlet.2014.09.085

Figure 2.

Figure 2

MDA-MB-231 cells were incubated with ca. 50 nM of the singly linked dimer 6b for 24 hours, rinsed four times with PBS buffer to remove any unbound dye and fixed with 4% paraformaldehyde solution in NaOH. Fluorescence images were captured using 540–580 nm band pass excitation and 600–650 nm band pass emission, magnification 20× under identical instrumental conditions. (A) Just after preparation of the fixed cells, and (B) the same slide after 3 days. The contrast in each case was enhanced by 40% for publication.