Figure 3.
Top: after treating MDA-MB-231 human breast cancer cells with 5b and 6b for 24 hours and removing the unbound dye, dark toxicity of 5b (blue), 6b (green) and phototoxicity of 5b (red) and 6b (orange) upon irradiation of cells with white light (0.92 mW cm−2; 11.04 kJ m−2 for 20 min) were determined using a trypan blue assay. Bottom: the cells continue to become necrotic for the next 18 hours after similar treatment with 10 μM of 5b (black) and 6b (brown).