Figure 6.

Compound S1 inhibition of ERK phosphorylation.

Notes: (A) Macrophages were cultured with or without S1–S5 or AS29 for 0.5 hour and stimulated with LPS (0.5 μg/mL) for a further 20 minutes. Cells were harvested and the total protein was extracted. The protein levels of p-ERK, ERK, p-JNK, JNK, p-P38 and P38 were determined by Western blot. (B) S1 dose-dependently decreased ERK phosphorylation. Representative blots of three independent experiments in each study are shown. Statistical significance with regard to LPS was demonstrated: *P<0.05, **P<0.05, and ***P<0.001, respectively.

Abbreviations: Comp, compound; LPS, lipopolysaccharide; ERK, extracellular regulated protein kinase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; JNK, c-Jun N-terminal kinase; P, phosphorylation; SEM, standard error of the mean.