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. 2014 Nov 10;9(11):e110411. doi: 10.1371/journal.pone.0110411

Figure 3. TBr inhibited STATs signaling in leukemia cell lines.

Figure 3

(A) Cells were treated with TBr (3 µM) at indicated time intervals and protein lystaes were prepared for immunoblotting of indicative proteins (B) Concentration dependent inhibition of p-STAT3 (Y705 and S727) in TBr treated HL-60 cells for 6 h. (C) TBr inhibited STATs signaling in MOLT-4 and K-562 cells, when treated for 6 h at indicated concentrations. β actin was used as a loading control. (D) Immunofluorescence of p-STAT3 (Y705) in TBr (3 µM) treated HL-60 cells. TBr treatment significantly decreased p-STAT3 (Y705) expression as indicated by the decrease in green fluorescence. (E) TBr inhibited IL-6 induced STAT3 phosphorylation. HL-60 cells were pre-treated with TBr (3 µM) at indicated time intervals and IL-6 (50 ng/ml) was added 30 min before experiment termination to induce p-STAT3 expression.