Skip to main content
. 2014 Nov 10;9(11):e112290. doi: 10.1371/journal.pone.0112290

Figure 5. The in vitro and in vivo assays for FB1 adsorption by NSP.

Figure 5

(A) A muddy phenomenon (arrow) was noticed after mixing FB1 and NSP. Ctrl, tube containing DDW; NSP, tube containing 100 µg/mL of NSP; F20, tube containing 500 µg of FB1; FN20, tube containing 500 µg of FB1+100 µg of NSP. (B) After 2 h incubation, the supernatant collected from the mixture in each treatment was assayed for the concentration of FB1 using MaxSignal Fumonisin ELISA Test Kit, and the adsorption of FB1 with NSP showed a significant non-linear relationship. (C) The 16 pregnant female mice were divided into four groups and were consumed 0.2 mL of DDW (Ctrl), 100 µg of NSP (NSP), 500 µg of FB1 (F20), or 500 µg of FB1+100 µg of NSP (FN20) on days 7.5 and 8.5 of pregnancy. The plasma was collected from blood drawn through the tail vein 1 h after the last consumption, and the concentrations of FB1 were analyzed using the MaxSignal Fumonisin ELISA Test Kit. *: P<0.05 (Duncan's multiple range test).