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. 2014 Nov 10;9(11):e110787. doi: 10.1371/journal.pone.0110787

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© 2014 Wu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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BM CD3⁺ T cells from healthy individuals and SAA patients were co-cultured with mitomycin C treated autologous or allogenetic mononuclear cells for a total of 7 days, kinetics of sCD30 (A) and IFN-γ (B) released into culture supernatants were analysed by ELISA. Data represent mean ± SE of three independent experiments. (C) and (D) Released sCD30 and IFN-γ by allogeneic stimulated T cells at day 4 were determined in 5 healthy individuals and 6 SAA patients.