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. 2014 Nov 10;9(11):e112485. doi: 10.1371/journal.pone.0112485

Figure 3. SIK1 enhances gastrin-induced transcription.

Figure 3

AGS-GR cells cotransfected with expression plasmids and reporter plasmids and treated with gastrin (4 h). Results show one representative of three independent experiments; mean ± SD of six technical replicates. A: Cells co-transfected with c-fos-luc and pIRES (control) or pSIK1. B: The effect of pSIK1 wt and pSIK1 mutants on c-fos-luc expression. C: Cells co-transfected with CRE-luc and pIRES (control) or pSIK1. D: Cells cotransfected with pSIK1 and SRE-luc, AP1-luc or NF-κB-luc. E–F: AGS-GR cells (E) or MKN45 cells (F) transfected with pSIK1 or pIRES (control) and treated with gastrin. Phospho-HDAC4/5/7 was determined by Western blot. For both Western blots one representative of at least two independent biological experiments is shown. G: Intracellular localization of endogenous HDAC4/5/7. Upper panel; untreated cells, lower panel; gastrin treated cells (2 h). H: Intracellular localization of HDAC5 Flag. Upper panel; untreated cells, lower panel; gastrin treated cells (2 h).