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. 2014 Nov 10;9(11):e112466. doi: 10.1371/journal.pone.0112466

Figure 12. Anterograde labeling from lumbosacral DRG in vivo reveals spinal afferent nerve endings in the mucosa of three different mice.

Figure 12

A, a single nerve axon ending is shown consisting of a bare unspecialized terminal with few varicosities. The arrows indicate the trajectory of this axon that is initially out of focus as it projects through the submucosa, then becomes in focus at the level of the mucosa. B, shows CGRP immunoreactivity of the region shown in panel A. The arrows indicate the same region of the axon that is in panel A. C, shows a superimposed image of panels B & A. The arrows indicate the anterogradely labeled axon is CGRP immunoreactive. D, shows a mucosal ending in a different animal consisting of a bifurcating axon, again with very few varicosities along its axon (see arrow). E, shows CGRP immunoreactivity of the region shown in panel D, see arrow. F, shows a superimposed image of panels E and D. This ending is CGRP positive, see arrow. G, shows another example from a different mouse of a varicose spinal afferent ending in the mucosa. The arrow shows a fine varicose ending. H, shows CGRP immunoreactivity of the region shown in G. The arrow indicates a lack of CGRP immunoreactivity of the ending shown by the arrow in panel G. I, shows a superimposed image of panels G and H. It can be seen that this mucosal ending is not CGRP positive (see arrows).