Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1973 Feb;7(2):190–195. doi: 10.1128/iai.7.2.190-195.1973

Inhibition of Migration of Mouse Macrophages by Tuberculin-Sensitive Mouse Lymphocytes and by Mouse Migration Inhibitory Factor

Rochelle G Neiburger 1, Guy P Youmans 1
PMCID: PMC422658  PMID: 4633290

Abstract

The guinea pig migration inhibition technique, an accepted in vitro correlate of delayed hypersensitivity, has been adapted to a murine system. Peritoneal exudate cells from CF-1 mice vaccinated with viable cells of the H37Ra strain of Mycobacterium tuberculosis were inhibited in vitro by purified protein derivative (PPD) or whole H37Ra microorganisms. Peritoneal exudate cells from the inbred C57Bl/6 mice immunized with H37Ra cells also were inhibited in vitro by PPD or whole H37Ra microorganisms. Migration inhibitory factor (MIF) was produced by splenic lymphocytes from the H37Ra-immunized C57Bl/6 mice when incubated with either antigen. Intravenous injection of PPD or viable H37Ra organisms into H37Ra mice resulted in MIF production in vitro by splenic lymphocytes without further antigenic stimulation. Peritoneal exudate cells from nonimmunized C57Bl/6 mice and supernatant fluids from cultures of lymphocytes from nonimmunized C57Bl/6 mice were not inhibited in the presence of antigen. The production of MIF by splenic lymphocytes from immunized C57Bl/6 mice depended upon the conditions under which the lymphocytes were cultured, the time of exposure to antigen (3 days), the use of a higher concentration of PPD for stimulation of lymphocytes than that required for guinea pig cells, and also the use of cells from a highly inbred mouse strain.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AL ASKARI S., DAVID J. R., LAWRENCE H. S., THOMAS L. IN VITRO STUDIES ON HOMOGRAFT SENSITIVITY. Nature. 1965 Feb 27;205:916–917. doi: 10.1038/205916a0. [DOI] [PubMed] [Google Scholar]
  2. Bloom B. R., Bennett B. Mechanism of a reaction in vitro associated with delayed-type hypersensitivity. Science. 1966 Jul 1;153(3731):80–82. doi: 10.1126/science.153.3731.80. [DOI] [PubMed] [Google Scholar]
  3. CHANG Y. T. LONG-TERM CULTIVATION OF MOUSE PERITONEAL MACROPHAGES. J Natl Cancer Inst. 1964 Jan;32:19–35. [PubMed] [Google Scholar]
  4. Collins F. M., Mackaness G. B. The relationship of delayed hypersensitivity to acquired antituberculous immunity. I. Tuberculin sensitivity and resistance to reinfection in BCG-vaccinated mice. Cell Immunol. 1970 Sep;1(3):253–265. doi: 10.1016/0008-8749(70)90047-x. [DOI] [PubMed] [Google Scholar]
  5. DAVID J. R., AL-ASKARI S., LAWRENCE H. S., THOMAS L. DELAYED HYPERSENSITIVITY IN VITRO. I. THE SPECIFICITY OF INHIBITION OF CELL MIGRATION BY ANTIGENS. J Immunol. 1964 Aug;93:264–273. [PubMed] [Google Scholar]
  6. David J. R. Delayed hypersensitivity in vitro: its mediation by cell-free substances formed by lymphoid cell-antigen interaction. Proc Natl Acad Sci U S A. 1966 Jul;56(1):72–77. doi: 10.1073/pnas.56.1.72. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Feinstone S. M., Beachey E. H., Rytel M. W. Induction of delayed hypersensitivity to influenza and mumps viruses in mice. J Immunol. 1969 Oct;103(4):844–849. [PubMed] [Google Scholar]
  8. GEORGE M., VAUGHAN J. H. In vitro cell migration as a model for delayed hypersensitivity. Proc Soc Exp Biol Med. 1962 Nov;111:514–521. doi: 10.3181/00379727-111-27841. [DOI] [PubMed] [Google Scholar]
  9. Galindo B., Myrvik Q. N. Migratory response of granulomatous alveolar cells from BCG-sensitized rabbits. J Immunol. 1970 Jul;105(1):227–237. [PubMed] [Google Scholar]
  10. MACKANESS G. B. The action of drugs on intracellular tubercle bacilli. J Pathol Bacteriol. 1952 Jul;64(3):429–446. doi: 10.1002/path.1700640302. [DOI] [PubMed] [Google Scholar]
  11. Ranlov P., Hardt F. The migration-inhibitory factor (MIF). Effect of antilymphocytic serum on formation and performance of MIF in murine in vitro experiments. Transplantation. 1970 Nov;10(5):438–442. [PubMed] [Google Scholar]
  12. Sandok P. L., Hinsdill R. D., Albrecht R. M. Migration inhibition of mouse macrophages by Brucella antigens. Infect Immun. 1971 Oct;4(4):516–518. doi: 10.1128/iai.4.4.516-518.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Schlossman S. F., Levin H. A., Rocklin R. E., David J. R. The compartmentalization of antigen-reactive lymphocytes in desensitized guinea pigs. J Exp Med. 1971 Sep 1;134(3 Pt 1):741–750. doi: 10.1084/jem.134.3.741. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Taubler J. H., Mudd S. Staphylococcal delayed hypersensitivity in mice. II. In vitro demonstration and specificity of delayed hypersensitivity. J Immunol. 1968 Sep;101(3):550–555. [PubMed] [Google Scholar]
  15. Yamamoto K., Anacker R. L. Macrophage Migration Inhibition Studies with Cells from Mice Vaccinated with Cell Walls of Mycobacterium bovis BCG: Characterization of the Experimental System. Infect Immun. 1970 Jun;1(6):587–594. doi: 10.1128/iai.1.6.587-594.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES