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. 2014 Jul 9;177(2):381–390. doi: 10.1111/cei.12336

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Fig. 3 — Effect of [Nle4, DPhe7]-α-MSH (NDP-MSH on anti-nuclear and anti-cytoplasmic plasma antibodies (ANA). ANA was detected by indirect immunofluorescence using human epithelial type 2 (HEp-2) cells home-made assay. (a) Negative pattern, (b) positive pattern of ANA (original magnification ×400). ANA was detected in all lupus mice (LM) 180 days after pristane lupus induction (c), whereas only 30% (three of 10) of LM treated with NDP-MSH (LM-MSH) had ANA. *P < 0·05 compared with control animals, #P < 0·05 compared with LM.