Table 1.
Human leucocyte antigen (HLA) types (a) and HLA immunoglobulin (Ig)G alloantibodies (b) in the serum of a woman alloimmunized postpartum 23 years ago. (a) HLA genotype of an alloimmunized woman and her family. (b) HLA class II IgG alloantibodies in the serum of the alloimmunized woman. The HLA class II allele found in the husband and first child was DRB1*0101, against which IgG antibody was found both in the serum and culture supernatant. The serum antibody levels are expressed as mean fluorescent intensity (MFI) after correcting against negative control (MFI: < 80). The antibodies found in wife's serum against the specific alleles (DRB1*0101 and DQA1*0101/DQB1*0501) of the husband (shown in bold type) are referred to as primary alloantibodies. The primary alloantibodies may cross-react with closely related alleles, resulting in secondary alloantibodies. Asterisks indicate the antibodies found in the activated B cell culture. Only one primary antibody is found (shown as ***) and five different secondary antibodies with varying MFI (* or **) are present
| (a) Alleles shared by husband, wife and children with their ages | |||||
|---|---|---|---|---|---|
| HLA classes | Haplotype | Husband (53) and 1st child (23) | Husband (53) and 2nd child (18) | Wife (54) and 1st child | Wife (54) and 2nd child |
| I | A | *1101 | *3002 | *2402 | *3301 |
| B | *3501 | *2703 | *4801 | *5801 | |
| Cw | *0410 | *0327 | *0302 | *0803 | |
| II | DRB1 | 1*0101 | 1*1501 | 1*1302 | 1*1501 |
| DRB5 | 5*0101 | 5*0101 | 5*0101 | 3*0301 | |
| DQA1 | 1*0101 | 1*0102 | 1*0505 | 1*0102 | |
| DQB1 | 1*0501 | 1*0602 | 1*0609 | 1*0301 | |
| DPA1 | 1*0202 | 1*0103 | 1*0103 | 1*0202 | |
| DPB1 | 1*0402 | 1*0501 | 1*0501 | 1*0301 | |
| (b) Anti-HLA-IgG antibodies (expressed in MFI) found in the serum of a woman 23 years after postpartum alloimmunization | ||||||
|---|---|---|---|---|---|---|
| Alleles present in the husband and 1st child | Alleles recognized by the IgG antibodies | Serum dilutions | IgG detected in vitro | |||
| (1:10) | (1:20) | (1:40) | (1:80) | |||
| (+) | DRB1*0101 | 4 912 | 2785 | 1415 | 709 | *** |
| DRB1*0102 | 4 320 | 2231 | 1179 | 612 | ** | |
| DRB1*1001 | 2 276 | 1161 | 530 | < 500 | ||
| DRB1*0404 | 1 860 | 971 | 513 | < 500 | * | |
| DRB1*0103 | 1 290 | 615 | < 500 | < 500 | ||
| DRB4*0101 | 1 179 | 747 | 515 | < 500 | ||
| DRB1*0401 | 971 | 519 | < 500 | < 500 | * | |
| DRB1*0405 | 970 | 500 | < 500 | < 500 | * | |
| DRB1*1402 | 933 | 500 | < 500 | < 500 | * | |
| DRB1*1401 | 859 | < 500 | < 500 | < 500 | ||
| DRB1*0403 | 608 | < 500 | < 500 | < 500 | ||
| (+) | DQA1*0101\DQB1*0501 | 12 153 | 7814 | 4507 | 2483 | |
| DQA1*0102\DQB1*0502 | 7 106 | 4335 | 2443 | 1313 | ||
| DQA1*0501\DQB1*0201 | 5 677 | 4145 | 2395 | 1265 | ||
| DQA1*0401\DQB1*0201 | 538 | < 500 | < 500 | < 500 | ||