Figure 2.

Lung deflation and reinflation promoted differential phosphorylation of p38-mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinase 1 and 2 (ERK1/2), and Akt. The lungs were subjected to 20 minutes of equilibration (baseline, 0 minutes), followed by deflation (60 minutes) and reinflation and reperfusion (60 minutes). The tissue samples were collected at the points indicated. Whole cell extracts were immunoblotted for phosphorylated (p)-p38-MAPK, p-ERK, or p-Akt. Equal protein loading was verified by immunoblotting for total p38-MAPK. The blots were quantified using scanning densitometry. The results were normalized to the baseline measurements and are expressed as the mean ± standard error of the mean of 4 lungs per group. *P < .05 compared with baseline (1-way analysis of variance with Dunnett's test). Bas, Baseline.