Skip to main content
. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Mol Cancer Ther. 2014 Mar 31;13(6):1578–1588. doi: 10.1158/1535-7163.MCT-13-0877

Figure 1. PELP1 is needed for optimal activation of the mTOR pathway.

Figure 1

(A, B) MCF7 and ZR75 cells were transiently transfected with nonspecific control siRNA or PELP1 specific siRNA for 72 h, serum starved for 24h and stimulated with 10% serum for 10 min. Status of phosphorylation of mTOR signaling components was analyzed by Western blotting. Quantitation of phosphorylation after normalizing to its respective total protein is shown.(C) Lysates of MCF7 breast cancer cells were subjected to immunoprecipitation with PELP1, Raptor, and Rictor antibodies, and the presence of mTOR and PELP1 in the immunoprecipitates was analyzed by Western blotting.