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. 2014 Jul 15;5(18):8341–8354. doi: 10.18632/oncotarget.2202

Figure 4. HMGA1P6 and HMGA1P7 expression increases cell proliferation and reduces apoptosis.

Figure 4

(A) and (B) HEK293 and 8505c cell proliferation in HMGA1P6- and HMGA1P7-transfected cells. (C) HEK293 cells were transfected with the control, HMGA1P6 or HMGA1P7 vectors. The DNA content of the transfected HEK293 cells was analyzed by flow cytometry after propidium iodine staining. (D) 8505c cell proliferation in siRNA-HMGA1P6- and siRNA-HMGA1P7-transfected cells. (E) HMGA1P6- and HMGA1P7-transfected cells were treated with doxorubicin, and apoptosis was assessed by FACS. (F) HEK293 cells were starved, and apoptosis was assessed by Western blot analysis of Caspase 9 cleavage. The results are reported as the mean of values; Error bars represent means ± SD; n =3. *, P < 0.05 **, P < 0.01 ***, P < 0.001 (t test).