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. 2014 Aug 6;5(18):8429–8441. doi: 10.18632/oncotarget.2316

Figure 3. ISG15 promotes cancerous proliferation, migration and involves in cell cycle.

Figure 3

ISG15 was knocked down in 97L cells (97L-shISG15) (A, left pannel) and was overexpressed in Huh7 cells (Huh7-ISG15) (A, right panel). β-actin was probed as a control. (B) 97L-shISG15 cells (left panel, P = 0.003) or Huh7-ISG15 cells (right panel, P = 0.007) were cultured from 24 to 72h followed by incubation with [3H]-thymidine for 4 h. Data are from three independent experiments with three dishes and shown as means ± SD. (C) 97L-shISG15 cells or Huh7-ISG15 cells were analyzed to detect respectively their cell cycle by flow cytometry using propidium iodide (PI) staining. (D) Cell lysates were immunoblotted with antibodies against Cyclin B1 or CDK1. β-actin was used as a loading control. (E) Trans-well migration assays showed that there was a positive correlation between ISG15 expression and HCC cell migration (**, P < 0.01). The HCC cell migration was shown in the microscopic fields.