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. 2014 Aug 6;5(18):8429–8441. doi: 10.18632/oncotarget.2316

Figure 4. ISG15 maintains Survivin protein stabilization via XIAP.

Figure 4

Survivin protein was detected using western blotting in 97L-shISG15 cells (A) or Huh7-ISG15 cells (B). β-actin was probed as a negative control. (C) Survivin mRNA was detected using realtime-PCR in 97L-shISG15 cells and Huh7-ISG15 cells. β-actin was probed as a negative control. (P > 0.05). (D) Examination of Survivin protein degradation pathways. 97L cells were incubated with either a proteasome inhibitor (MG-132) or a lysosomal inhibitor (chloroquine). (E) Proteins from lysates were immunoprecipitated with antibody to Flag, and followed by immunoblotting with antibodies to myc. (F) Proteins from lysates were immunoprecipitated with antibody to Flag, and followed by immunoblotting with antibodies to HA, Flag and myc. (G) A model chart described the interaction between ISG15, Survivin and XIAP.