Skip to main content
. 2014 Aug 6;5(18):8442–8451. doi: 10.18632/oncotarget.2319

Figure 3. Rapamycin-induced phosphorylation of eIF4E is independent of the MAPK pathways.

Figure 3

Daoy cells were pretreated with DMSO or inhibitors for MEK (U0126, 10 μM), p38 (SB203580, 20 μM), Rsk1 (BI-D1870, 10 μM), for 60 min before addition of rapamycin (20 nM) for 2 hours. Equal amounts of protein were resolved by SDS-PAGE and processed by immunoblotting using antibodies for the phosphorylated forms of eIF4E (pSer-209), p70-S6K (pSer-389) and Akt (pSer-473). The blots were stripped and reprobed with antibodies for eIF4E, p70-S6K, Akt and GAPDH, as indicated.