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. 2014 Aug 8;5(18):8466–8477. doi: 10.18632/oncotarget.2327

Figure 1. PIWIL2 alters c-Myc expression in tumor cells.

Figure 1

(A) PIWIL2 upregulates c-Myc expression at protein level. HeLa or HepG2 cells were transfected with indicated plasmids and harvested for Western blot analysis. (B) PIWIL2 upregulates c-Myc expression at mRNA level. RT-qPCR assay was performed and fold change was normalized by control to an arbitrary value of one. The results were presented as mean ± s.d. (n=3). *, P<0.05. NS, not significant. (C) Immunofluorescent staining of c-Myc in transfected cells with PIWIL2 expression constructs or shRNA expression vectors. HeLa and HepG2 cells were fixed, permeabilized and incubated with c-Myc primary antibodies and Cy3-labelled secondary antibodies (red) for c-Myc staining 48h after transfection. DAPI (blue), nucleus. Scale bar, 20μm.