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. 2014 Aug 8;5(18):8466–8477. doi: 10.18632/oncotarget.2327

Figure 5. PIWIL2 can enhance cell proliferation and cell cycle progress via c-Myc.

Figure 5

(A) Cell Counting Kit-8 assay showed that c-Myc knockdown abates impact of PIWIL2 on cell proliferation. (B) c-Myc overexpression recovers impact of PIWIL2 knockdown on cell proliferation. (A) And (B) HeLa or HepG2 cells were transfected with various constructs for overexpression or knockdown by shRNA respectively. Cell proliferation was analyzed by Cell Counting Kit-8 assays in the indicated time. The relative proliferation was presented as fold change that was calculated by absorbance and normalized by control to an arbitrary value of one. (C) NME2 and PIWIL2 enhance cell cycle progress. (D) c-Myc is involved in PIWIL2 induced the entry of tumor cells from G0/G1 into S-phase. (C) And (D) HeLa or HepG2 cells were transfected with various constructs for overexpression or knockdown by shRNA respectively. After transfection, cells were stained with propidium iodide and the cell cycle was analyzed using flow cytometry. The results were presented as mean ± s.d. (n=3). *, P<0.05. NS, not significant.