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. 2014 Aug 10;5(18):8583–8601. doi: 10.18632/oncotarget.2323

Figure 1. Induction of genomic instability in NPC cells with EBV BALF3 expression.

Figure 1

TW01 cells were transiently transfected with the doses of pEGFP-C1 or pEGFP-C1-BALF3 indicated for 24 h, followed by western blotting with antibodies specific to GFP and GAPDH (A) and micronucleus assay (B). Data are presented as means ± standard deviations. Student's t test was used to determine the difference between two groups. *, P < 0.05; **, P < 0.001. Mock, mock transfection; MN, micronucleus. (C and D) TW01 cells were transiently transfected with 0.8 μg pEGFP-C1, pEGFP-C1-BALF3 or pEGFP-C1-G624A for 24 h prior to western blotting and micronucleus assay. Data are presented as means ± standard deviations. Student's t test was used to determine the difference between two groups. *, P < 0.05; **, P < 0.01. Mock, mock transfection; MN, micronucleus. (E and F) HONE-1 cells were transiently transfected with the doses of pEGFP-C1, pEGFP-C1-BALF3 or pEGFP-C1-G624A indicated for 24 h, following by western blotting and micronucleus assay. Data are presented as means ± standard deviations. Student's t test was used to determine the difference between two groups. *, P < 0.05. Mock, mock transfection; MN, micronucleus. (G) TW01 and HONE-1 cells were transiently transfected with 0.8 μg pEGFP-C1, pEGFP-C1-BALF3 or pEGFP-C1-G624A for 24 h prior to indirect immunofluorescence staining with an antibody specific to γH2AX. The nuclei of the cells were stained with Hoechst33258. Mock, mock transfection.