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. 2014 Aug 13;5(18):8614–8624. doi: 10.18632/oncotarget.2345

Figure 1. Expression of CD146 on endothelium in murine and human hepatocellular carcinoma.

Figure 1

(A, B) Representative images of immunofluorescence labeling with Alexa Fluor 488–conjugated anti-Lyve-1 (green) and PE-conjugated anti-CD146 antibodies (red). LSCM of histological slides (A) and whole-mount tissue after intravenous injection (B). Lyve-1 was strongly expressed by normal sinusoidal endothelial cells. High levels of CD146 staining were found in all tumor blood vessels, in microvessels of the periportal area, and in zone 1 of the acinus in the liver. T, tumor; L, liver. (C) Image-based analysis of CD146 staining on histological slides. We observed a high mean fluorescence intensity of CD146 on tumor blood vessels. (D–F) Comparison of CD146 expression in isolated HECs and TECs. mRNA levels (D), representative fluorescence staining with Alexa Fluor 488–ME-9F1 mAb (E), and ELISA of cell lysates (F). CD146 expression was significantly higher in TECs compared to HECs (P<0.05). (G, H) Expression of CD146 on endothelium in human tissue; immunohistochemical staining of CD146. (G): Representative images of snap-frozen tissue, L, liver tissue, C, connective tissue. (H): Sample distribution according to expression intensity, formalin-fixed samples of 41 tumors and 3 livers were included into the analysis. CD146 was overexpressed in the majority of human HCC samples.