Skip to main content
. 2014 Aug 17;5(18):8665–8680. doi: 10.18632/oncotarget.2356

Figure 4. The effect of Withaferin A analogues on the expression of phenotypic CSC properties.

Figure 4

(A) Chemical structures of Withaferin A, Withanone, Withanolide A and 12-Deoxywithastramonolide (12-DWS). (B, C) iCSCL-10A cells were treated with 1 μM of Withaferin A, Withanone, Withanolide A, or 12-DWS for 48 hrs followed by the ALP assay. Phase contrast images of ALP staining (B). Values represent the mean ± SEM (n = 3) (C). Scale bar, 500 mm. (D, E) iCSCL-10A cells treated with 1 μM of Withaferin A, Withanone, Withanolide A and 12-DWS for 48 hrs and the tumor spheres were enumerated. Phase contrast images of tumor spheres are shown (D). Scale bar, 500 mm.Values represent the mean ± SEM (n = 3, E).