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. 2014 Oct 20;5(18):8765–8777. doi: 10.18632/oncotarget.2364

Figure 2. IL-32α interacts simultaneously with BCL6 and PKCε in the presence of PMA.

Figure 2

(A) HEK293 cells were transfected with FLAG-tagged BCL6. Cells were incubated overnight then treated with 50 nM PMA for 3h. (B) HEK293 cells were cotransfected with vectors expressing FLAG-tagged BCL6, FLAG-tagged PKCε, and Myc-tagged IL-32α. After cotransfection, HEK293 cells were treated with the pan-PKC inhibitor, Gö6850 (6850; 10 μM), for 2 h followed by 50 nM PMA for 6 h. (C) The interactions were also observed between endogenous PKCε, IL-32α, and BCL6. HEK293 cells were cotransfected with FLAG-tagged BCL6 and Myc-tagged IL-32α as indicated. HEK293 cells were pretreated with a pan-PKC inhibitor, Gö6850 (6850; 10 μM), for 2 h then treated with 50 nM PMA for 6 h. Immunoprecipitations with 1 μg of anti-PKCε or normal IgG antibody were performed, and the precipitated IL-32α and BCL6 was detected. Immunoprecipitation with normal rabbit IgG antibodies (IgG) was used as a control.