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. 2014 Nov 10;207(3):419–432. doi: 10.1083/jcb.201404107

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© 2014 Shen et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 1. — Theoretical optimization of pH sensor properties. (A) Theoretical calculation of optimal pK_a for the largest fluorescence change from 5.5 to 7.5, with a n_H of 1.0. Black solid line, relative fluorescence at pH 7.5 (F_7.5/F_max); black dashed line, relative fluorescence at pH 5.5 (F_5.5/F_max); red line, fluorescence fold change (F_7.5/F_5.5); blue line, relative fluorescence intensity change ((F_7.5-F_5.5)/F_max); magenta line, intensity scaled fold change, which is the multiplication product of fold change and intensity change. (B) Effect of n_H on the intensity scaled fold change at different pK_a values. The pH-sensitive FPs covered in this work are mapped according to pK_a and n_H values. From top to bottom (n_H descending), right to left (pK_a descending): SEP; pHuji; pHoran4 (orange, same as mOrange and mOrange variants), pHoran3, A-17 (blue, same as mApple and mApple variants), pHoran2, and pHoran1; A-47, mNectarine (white), and mOrange; mCherry-TYG (black), A-9, and mApple; and pHTomato.