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. 2014 Nov 10;207(3):375–391. doi: 10.1083/jcb.201404016

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© 2014 Ogun and Zallocchi

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 4. — FM1-43 uptake experiments. (A–H) Representative images showing dye uptake assessed at 2 dpf (A–D) and 3 dpf (E–H) larvae. (A and E) Control. (B and F) Clarin-1 (Clrn1) MOs. (C and G) MOs + cRNA. (D and H) MOs + cRNAΔCT. Hair cell bundle was counterstained with phalloidin (green). Bar, 7 µm. (I) Semiquantitative analysis of the total fluorescence per neuromast. (J) Number of hair cells per neuromast. (K) Fraction of FM1-43–positive hair cells. Results are expressed as means ± SEM. Number of neuromasts analyzed from independent samples, n > 15. *, P < 0.05; **, P < 0.01; ***, P < 0.001 versus control MO. ++, P < 0.01; +++, P < 0.001 versus clarin-1 MO + cRNA. a.u., arbitrary unit.