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. 2014 Nov 10;207(3):407–418. doi: 10.1083/jcb.201406032

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© 2014 Löchte et al.

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Figure 7. — STAT activation at micropatterned signaling complexes. (a) U5A cells stably expressing SNAP-IFNAR1 and HaloTag-IFNAR and transiently transfected with STAT1-EGFP (green channel) and STAT2-tagRFP (yellow channel) imaged after stimulation with ^AT655IFNα2 (red channel). (bottom center/right) Nuclear translocation of STAT1 and STAT2 20 min after incubation with IFNα2. Cell boundaries are indicated by dotted lines. Bars, 10 µm. (b) Changes in the fluorescence intensity observed for STAT1 and STAT2 within micropatterns compared for stimulated and nonstimulated cells (mean intensity of five ROIs, representative of three cells analyzed). rel., relative.