Effects of fasting on hepatic gene expression of ghr2 (A), igf1 (B), igf2 (C), igfbp1b (D), igfbp2b (E) and igfbp5a (F). Tilapia were exposed to continuous feeding (open bars) or fasting (solid bars) and sampled at 7, 14 and 28 days. Significant effects of time or interaction between time and fasting are indicated in respective panels. When there was a significant interaction between time and fasting, post-hoc comparisons were made between fed and fasted groups at each time point. *P < 0.05, **P < 0.01 and ***P < 0.001. Means ± SEM (n = 7–8).