Figure 1. Characterization of the ADRB response profile.

PMN responses toward purified IgGs obtained from Ghanaian SIP samples were analyzed using the sADRB (A and C) and mADRB (B and D) assays over a 60-min kinetic window. (A and B) One representative sample (SIP 27, black circles) is shown, which elicits a typical sADRB kinetic profile using MSP-1₁₉ as the antigen (A) and a typical mADRB kinetic profile (B) using PMNs from one donor. IgG from a European NIP (black squares) and PBS (black triangles) were used as negative controls. The influence of complement in the sADRB (C) and mADRB (D) assays was assessed using native plasma (open diamonds), decomplemented plasma (black squares), and purified IgG (black circles), using mixed PMNs from two donors. The response profiles toward the IgG/plasma from SIP 5 are shown as a representative example. Profiles represent the mean of the detected luminescence intensity in relative luminescence units (rlu) ± range of one technical duplicate.