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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: J Vasc Surg. 2014 May 10;62(4):1040–1047.e2. doi: 10.1016/j.jvs.2014.04.033

Fig 1. Lysophosphatidylcholine (lysoPC) induces externalization of canonical transient receptor potential (TRPC) 6 protein in TRPC5-/- endothelial cells (ECs).

Fig 1

(A) Wild-type and TRPC5-/- ECs were incubated with or without lysoPC for 1 hour. Cell surface proteins were biotinylated and immunoblot analysis was performed for biotinylated TRPC6 (top panel). Prior to incubation with streptavidin-agarose beads, an aliquot of cell lysate was removed for immunoblot analysis to determine total TRPC6 protein level (bottom panel). (B) WT or TRPC5-/- ECs were incubated with or without lysoPC for 15 minutes, and then exposed to anti-TRPC6 antibody followed by Alexa 488 conjugated secondary antibody. TRPC6 location was assessed by fluorescence microscopy. Nuclei were detected by counterstaining with propidium iodide. Original magnification, x40. Bar, 40 μm.