Table 1.
qRT-PCR primer pair sequences for selected genes
| Gene | Accession number | Forward primer | Reverse primer |
|---|---|---|---|
| Alb | NM_009654.3 | CAAGAGTGAGATCGCCCATCG | TTACTTCCTGCACTAATTTGGCA |
| Cxcl12 | NM_013655.4 | TGCATCAGTGACGGTAAACCA | TTCTTCAGCCGTGCAACAATC |
| Gapdh | NM_008084.2 | AGGTCGGTGTGAACGGATTTG | TGTAGACCATGTAGTTGAGGTCA |
| GusB | NM_010368.1 | CCGACCTCTCGAACAACCG | GCTTCCCGTTCATACCACACC |
| Mmp1a | NM_032006.3 | CCTTGATGAGACGTGGACCAA | ATGTGGTGTTGTTGCACCTGT |
| Pdgfa | NM_008808.3 | CGATGAGGACCTGGGCTT | TTCTCGGGCACATGGTTAATG |
| Tgfb2 | NM_009367.3 | AGAATCGTCCGCTTTGATGTC | TCTGGTTTTCACAACCTTGCT |
| Tgfb3 | NM_009368.3 | CCTATCAGGTCCTGGCACTTT | CTGCCCGGAACAGATTGGT |
| THOC5 | NM_172438.3 | CTGTGTGCACTTCATGACTCTAAAGA | GAACTCCAGACATTTGGTGATCTCCT |
| Wnt11 | NM_009519.2 | GGTGGTACACCGGCCTATG | TCACTGCCGTTGGAAGTCTTG |
Alb: albumin (131 nt); Cxcl12: chemokine (C-X-C motif) ligand 12 (146 nt); Gapdh: glyceraldehyde-3 phosphate dehydrogenase (123 nt); GusB: glucuronidase, beta (169 nt); Mmp1a: matrix metallopeptidase 1a (238 nt); Pdgfa: platelet derived growth factor, alpha (232 nt); Tgfb2: transforming growth factor, beta 2 (198 nt); Tgfb3: transforming growth factor, beta 3 (252 nt); THOC5: THO complex subunit 5 homolog (178 nt), Wnt11: wingless-related MMTV integration site 11 (183 nt); (): product size.