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. 2014 Jul 4;11:55. doi: 10.1186/1742-4690-11-55

Table 1.

Infection of red-tailed (RT) guenons with a novel SIV in Kibale National Park, Uganda 1,2

Animal
 Sex
 HIV-2 WB
 HIV-1/2 InnoLIA
 SIV deep-sequencing 3 , RT-PCR results 4
    Gag Pol Env Gag Pol Env  
RT01
 F
 -
 -
 -
 -
 -
 -
 -
RT02
 M
 -
 -
 -
 -
 -
 -
 -
RT03
 F
 -
 -
 -
 -
 -
 -
 SIVrtg_Kib + (9473)
RT04
 F
 w (p26)
 -
 w (gp80)
 -
 -
 -
 -, RT-PCR +
RT05
 F
 + (p26)
 -
 + (gp80)
 1+ (p24)
 -
 3+ (gp36)
 -, RT-PCR +
RT06
 M
 -
 -
 -
 -
 -
 -
 -
RT08
 F
 + (p26)
 -
 w (gp80)
 3+ (p24)
 -
 3+ (gp120)
 SIVrtg_Kib + (1932)
RT09
 M
 w (p26)
 -
 -
 -
 -
 -
 -
RT10
 M
 -
 -
 -
 -
 -
 -
 -
RT11
 F
 -
 -
 w (gp80)
 -
 -
 -
 SIVrtg_Kib + (5618)
RT12
 M
 -
 -
 -
 -
 -
 -
 -
RT13 M - - - - - - -

1SIV infection was assessed by deep-sequencing of reverse transcribed viral RNA and with serological tests, including HIV-2-specific western blots (HIV-2 WB) and HIV InnoLIA assays.

2For HIV-2 WB and HIV-1/-2 InnoLIA, seroreactivity to specific viral proteins is shown in brackets. For the HIV-1/-2 InnoLIA assay, intensity of signals was quantified with "1+" being positive and "3+" being strongly positive. For the HIV-2 WB, intensity of signals was quantified with "w" being weak and "+" being positive.

3To quantify the number of SIV-specific reads, read numbers were normalized to 1 million reads per animal.

4A 400-bp fragment of the C-terminal half of the polymerase (pol) gene was amplified by RT-PCR and the presence of SIVrtg_Kib was subsequently confirmed by Sanger sequencing.